Journal: ACS Biomaterials Science & Engineering
Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation
doi: 10.1021/acsbiomaterials.5c02107
Figure Lengend Snippet: Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. rhamnosus GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.
Article Snippet: Lactobacillus rhamnosus GG (LGG, ATCC 53103, Microbiologics, Cat #01090P) was cultured in De Man, Rogosa, Sharpe (MRS) media (BD, Cat # 288130) at 37 °C.
Techniques: Bacteria, Cell Culture, Fluorescence