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probiotic lactobacillus rhamnosus gg atcc 53103  (ATCC)


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    ATCC probiotic lactobacillus rhamnosus gg atcc 53103
    Probiotic Lactobacillus Rhamnosus Gg Atcc 53103, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lactobacillus+rhamnosus/La+Bel/pm42124006-241-5-9
    Average 96 stars, based on 388 article reviews
    probiotic lactobacillus rhamnosus gg atcc 53103 - by Bioz Stars, 2026-09
    96/100 stars

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    Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. <t>rhamnosus</t> GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.
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    Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. <t>rhamnosus</t> GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.
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    Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. <t>rhamnosus</t> GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.
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    Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. <t>rhamnosus</t> GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.
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    Image Search Results


    Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. rhamnosus GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation

    doi: 10.1021/acsbiomaterials.5c02107

    Figure Lengend Snippet: Impact of commensal and pathogenic bacteria on planar crypts with and without a mucus layer. (A) Schematic of the cell culture timeline to form planar crypts for these assays. (B) Percentage propidium iodide positive area relative to that of Hoechst 33342 for the 2 bacteria cocultured with the epithelium with and without a mucus layer. (C) Fluorescence images of planar crypt arrays ( XY ) cocultured with and without L. rhamnosus GG or S. aureus . Propidium iodide (red) and Hoechst 33342 (blue). Scale bar = 500 μm; inset scale bar = 100 μm. N = 3 biological replicates were imaged, i.e., three arrays. Two-way ANOVA multiple comparisons by Tukey. ** p < 0.001; * p < 0.05, ns for P value >0.05.

    Article Snippet: Lactobacillus rhamnosus GG (LGG, ATCC 53103, Microbiologics, Cat #01090P) was cultured in De Man, Rogosa, Sharpe (MRS) media (BD, Cat # 288130) at 37 °C.

    Techniques: Bacteria, Cell Culture, Fluorescence